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  • Shoji-Kawata, S., Sumpter, R., Leveno, M., Campell, G.R., Zou, Z., Kinch, L., Wilkins, A.D., Sun, Q., Pallauf, K., MacDuff, D., Huerta, C., Virgin, H.W., Helms, J.B., Eerland, R., Tooze, S.A., Xavier, R., Lenschow, D.J., Yamamoto, A., King, D., Lichtarge, O., Grishin, N.V., Spector, S.A., Kaloyanova, D.V., Beth Levine (2013) Identification of a candidate therapeutic autophagy-inducing peptide. Nature 494, 201-206

  • Haberkant P, Raijmakers R, Wildwater M, Sachsenheimer T, Brügger B, Maeda K, Houweling M, Gavin AC, Schultz C, van Meer G, Heck AJ, Holthuis JC. (2013) In Vivo Profiling and Visualization of Cellular Protein-Lipid Interactions Using Bifunctional Fatty Acids. Angew Chem Int Ed Engl. In press

  • Brouwers, J., Aalberts, M., Jansen, J., van Niel, G., Stout, T., Helms, B., Stoorvogel, W. Distinct lipid compositions of two types of human prostasomes. (2013) Proteomics. In press.

  • Boerke, A., Brouwers, J.F., Olkkonen, V.M., van de Lest, C.H.A., Sostaric, E., Schoevers, E.J., Helms, J.B., B.M. Gadella. Involvement of Bicarbonate-Induced Radical Signaling in Oxysterol Formation and Sterol Depletion of Capacitating Mammalian Sperm During In Vitro Fertilization. (2013) Biology of Reproduction, In press.



  • Cebollero, E., van der Vaart, A., Zhao, M., Rieter, E., Klionsky, D.J., Helms, J.B., Reggiori, F. Phosphatidylinositol-3-phosphate clearance plays a key role in autophagosome completion. (2012) Current Biol. 22, 1545-1553

  • Van Galen, J., Olrichs, N., Schouten, A., Serrano, R.L., Note-'t Hoen, E., Eerland, R., Kaloyanova, D., Gros, P. J. Bernd Helms. Interaction of GAPR-1 with lipid bilayers is regulated by alternative homodimerization (2012) BBA-Biomembranes 1818, 2175–2183

    De Smet, C.H., Vittone, E., Scherer, M., Houweling, M., Liebisch, G., Brouwers, J.F., de Kroon A.I.P.M. The yeast acyltransferase Sct1p regulates fatty acid desaturation by competing with the desaturase Ole1p (2012) Mol. Biol. Cell 23, 1146-1156
  • Alejandro O. Sodero, Joris Vriens, Debapriya Ghosh, David Stegner, Marta Pallotto, Marco Sassoè-Pognetto, Bernhard Nieswandt, Jos Brouwers, Bernd Helms Thomas Voets and Carlos G. Dotti Cholesterol loss during glutamate-mediated excitotoxicity. EMBO J. 31, 1764 - 1773

  • Testerink, M., Ajat, M.A., Houweling, M., Brouwers, J.F., Pully, V.V., van Manen, H-J., Otto, C., Helms, J.B., and Vaandrager, A.B. Hepatic stellate cell activation induces formation of polyunsaturated TAG species. Raman microspectroscopy and lipidomic profiling of (activated) HSCs. PLoS ONE 7, e34945


  • Vogels, M.W., van Balkom, B.W.M., Kaloyanova, D.V., Batenburg, J.J., Heck, A.J., Helms, J.B.,  Rottier, P.J.M., de Haan, C.A.M. Identification of host factors involved in coronavirus replication by quantitative proteomic analysis (2011) Proteomics 11, 64-80.
  • Aardema, H., Vos, P.L.A.M., Lolicato F., Roelen, B.A.J. Knijn H.M., Vaandrager, A.B., Helms, J.B., Gadella, B.M. In vitro fatty acid exposure determines the developmental competence of bovine oocytes by modulating storage fat in lipid droplets (2011) Biology of Reproduction 85, 62-69.
  • Brouwers JF, Boerke A, Silva PF, Garcia-Gil N, van Gestel RA, Helms JB, van de Lest CH, Gadella BM Mass Spectrometric Detection of Cholesterol Oxidation in Bovine Sperm (2011) Biology of Reproduction 85, 128-136. 
  • Vogels, M.W., van Balkom, B.W.M., Heck, A.J., de Haan, C.A.M.,  Rottier, P.J.M., , Batenburg, J.J., Kaloyanova, D.V., Helms, J.B. Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle (2011) Proteomics 11, 4477-4491


  • Van der Meer-Janssen, Y.P.M., van Galen, J., Batenburg, J.J., and Helms, J.B. Lipids in host-pathogen interactions: pathogens exploit the complexity of the host cell lipidome (2010) Prog. Lipid Res. 49, 1-26 
  • Bot, M., Bot, I., Lopez-Vales, R., Van de Lest, C.H.A., Saulnier-Blache, J.S., Helms, J.B., David, S., Van Berkel, T.J.C., and Biessen, E.A.L. Atherosclerotic Lesion Progression Changes Lysophosphatidic Acid Homeostasis to Favor Its Accumulation (2010) Am. J. Pathol. 176, 3073-3084


  • Van de Wetering, K., Feddema, W., Helms, J.B., Brouwers, J.F., and Borst, P. Targeted metabolomics identifies glucuronides of dietary phytoestrogens as a major class of MRP3 substrates in vivo (2009) Gastroenterology 137, 1725-1735
  • Testerink, N., Van der Sanden, M.H.M., Houweling, M., Helms, J.B.,  and Vaandrager, A.B., Depletion of phosphatidylcholine affects endoplasmic reticulum morphology and protein traffic at the Golgi complex.  (2009) J. Lipid Res. 50, 2182-2206
  • Verheijen M. H.G., Camargo, N., Verdier, V., Nadra, K., de Preux, A., Medard, J., Luoma, A., Crowther, M., Inouye, H., Shimano, H., Chen, S., Brouwers, J.F., Helms, J.B., Feltri, M.L., Wrabetz, L., Kirschner, D., Chrast, R., Smit, A.B. SREBP cleavage activation protein (SCAP) is required for timely synthesis of a Schwann cell myelin membrane (2009) Proc. Natl. Acad. Sci. USA 106, 21383-21388


  • Retra, K., Bleijerveld, O.B., Gestel, R.A. van, Tielens, A.G.M., Hellemond, J.J. van, Brouwers, J.F.H.M. (2008). A simple and universal method for the separation and identification of phospholipid molecular species. Rapid Communications in Mass Spectrometry, 22, 1853-1862.


  • Li, X., Kaloyanova, D., van Eijk, M., Eerland, R., van der Goot, G., Oorschot, V., Klumperman, J., Lottspeich, F., Starkuviene, V., Wieland, F.T., and Helms, J.B. (2007) Involvement of a GPI-anchored Protein in Maintenance of the Golgi Structure. Mol. Biol. Cell 18, 1261-1271.
  • Huitema, L.F., van Weeren, P.R., van Balkom, B.W., Visser, T., van de Lest, C.H., Barneveld, A., Helms, J.B., and Vaandrager, A.B. (2007) Soluble factors released by ATDC5 cells affect the formation of calcium phosphate crystals. Biochim Biophys Acta. 1774,1108-1117.
  • Bleijerveld, O.B., Brouwers, J.F., Vaandrager, A.B., Helms, J.B., and Houweling, M. The CDP-Ethanolamine pathway and phosphatidylserine decarboxylation generate different phosphatidylethanolamine molecular species (2007) J. Biol. Chem. 282, 28362-28372.
  • Tsai, P.S., Vries, K.J. de, Boer-Brouwer, M. de, Garcia-Gil, N., Gestel, R.A. van, Colenbrander, B., Gadella, B.M. & Haeften, T. van  Syntaxin and VAMP association with lipid rafts depends on cholesterol depletion in capacitating sperm cells (2007) Molecular Membrane Biology, 24, 313-324.


  • Bleijerveld, O.B., Houweling, M., Thomas, M.J.H., & Cui, Z. (2006). Metabolipidomics: Profiling metabolism of glycerophospholipid species by stable isotopic precursors and tandem mass spectrometry. Analytical Biochemistry, 1, 1-14.
  • Helms, J.B. (2006). Host-Pathogen interactions: Lipids grease the way. European Journal of Lipid Science and Technology, 108, 895-897.
  • Helms, J.B., & Meer, G.F.B.P. van (2006). Lipidome and disease. FEBS letters, 580, 5429.


  • Moschetta, A., Xu, F., Hagey, L.R., Berge Henegouwen, G.P. van, Erpecum, K.J. van, Brouwers, J.F., Cohen, J.C., Bierman, M., Hobbs, H.H., Steinbach, J.H., & Hofmann, A.F.  (2005) A phylogenetic survey of biliary lipids in vertebrates. Journal of Lipid Research, 46(10), 2221-2232.


  • Helms, J.B. and Zurzolo, C. Lipids as targeting signals: lipid rafts and intracellular trafficking (2004) Traffic 5, 247-254.
  • Bleijerveld, O.B., Klein, W., Vaandrager, A.B., Helms, J.B. and Houweling, M. Control of the CDPethanolamine pathway in mammalian cells. Effect of CTP:phosphoethanolamine cytidyltransferase overexpression and the amount of intracellular diacylglycerol (2004) Biochem. J. 379, 711-719.
  • Serrano, R.L., Kuhn A., Hendricks A., Helms J.B., Sinning I., and Groves M.R. Structural analysis of the human Golgi-associated plant pathogenesis related protein GAPR-1 implicates dimerization as a regulatory mechanism (2004) J. Mol. Biol. 339, 173-183.

  • Van der Sanden, M.H.M., Meems, H., Houweling, M., Helms, J.B., and Vaandrager, A.B. Protein/Growth Arrest and DNA Damage-inducible Protein 153 Expression during Inhibition of Phosphatidylcholine Synthesis Is Mediated via Activation of a C/EBP-activating Transcription Factor-responsive Element (2004) J. Biol. Chem. 279, 52007-52015.

  • Huitema, K.R., Dikkenberg, J. van den, Brouwers, J.F.H.M., & Holthuis, J.C.M. (2004). Identification of a family of animal sphingomyelin synthases. EMBO Journal 23, 33-44.


  • Stüven, E., Porat, A., Shimron, F., Fass, E., Kaloyanova, D., Brügger, B., Wieland, F.T., Elazar, Z. and Helms, J.B. Intra-Golgi protein transport depends on a cholesterol balance in the lipid membrane. (2003) J. Biol. Chem. 278, 53112-53122.

  • Brouwers, J.F.H.M., & Gadella, B.M. In situ detection and localization of lipid peroxidation in individual bovine sperm cells. Relatoin to peroxidation of endogenous phosphatidylcholine and effects of storage (2003) Free Radical Biology and Medicine 35, 1382-1391

  • Sanden, M.H.M. van der, Houweling, M., Golde, L.M.G. van, & Vaandrager, A.B. Inhibition of phosphatidylcholine synthesis induces expression of the endoplasmic reticulum stress and apoptosis-related protein CCAAT/enhancer-binding protein-homologous protein (CHOP/GADD153) (2003) Biochemical Journal 369,  643-650.

  • Vries, K.J. de, Wiedmer, T., Sims, P.J., & Gadella, B.M. Caspase independent exposure of Aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cells (2003) Biology of Reproduction 68,  2122-2134


  • Eberle, H.B., Serrano, R.L., Füllekrug, J., Schlosser, M., Lehmann, W.D., Lottspeich, F., Kaloyanova, D., Wieland, F.T., and Helms, J.B. Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex. (2002) J. Cell Sci. 115, 827-838. 

  • Houweling M., W. Klein, M.J.H. Geelen. Regulation of phosphatidylcholine and phosphatidylethanolamine synthesis in rat hepatocytes by AMP-activated kinase. (2002) Biochem. J. 362 97-104 

  • Gadella B.M., R.A.P. Harrison. Capacitation induces cyclic adenosine 3', 5'-monophosphate-dependent, but apoptosis-unrelated, exposure of aminophospholipids at the apical head plasma membrane of boar sperm cells. Biol. Reprod. 67 (2002) 340-350